Genome analysis of the polysaccharide-degrading Echinicola strongylocentroti MEBiC08714

Abstract

Polysaccharides are a kind of natural polymeric carbohydrate molecules. These polysaccharides represent an important food source for heterotrophic organisms. Their biodegradation requires a set of enzymes that can cleave the glycosidic linkages of the carbohydrate backbone (called glycoside hydrolases) and the sulfate ester groups (called polysaccharide sulfatases). Echinicola strongylocentroti MEBiC08714T, which was isolated from a sea urchin Strongylocentrotus intermedius, has the ability to degrade seaweed polysaccharides. The genome consisted of 6.25 Mb with G+C content of 44.1 %. A total of 3337 genes were predicted, including 4,945 of protein coding genes, 43 tRNA genes and 12 rRNA genes. In addition, abundant putative enzymes involved in degrading polysaccharide were found. These enzymes include alginate lyase, amylase, agarase, ι-carrageenase, cellulase, fucosidase, pectate lyase, xylosidase as well as various sulfatases. These results reveal that Echinicola strongylocentroti MEBiC08714T have potential to degrade seaweed polysaccharides. The genome sequence of Echinicola strongylocentroti MEBiC08714T provides the fundamental genomic information for future studies on novel depolymeration enzymes produced by marine bacteria for utilizing seaweed for biofuels and chemicals production.ages of the carbohydrate backbone (called glycoside hydrolases) and the sulfate ester groups (called polysaccharide sulfatases). Echinicola strongylocentroti MEBiC08714T, which was isolated from a sea urchin Strongylocentrotus intermedius, has the ability to degrade seaweed polysaccharides. The genome consisted of 6.25 Mb with G+C content of 44.1 %. A total of 3337 genes were predicted, including 4,945 of protein coding genes, 43 tRNA genes and 12 rRNA genes. In addition, abundant putative enzymes involved in degrading polysaccharide were found. These enzymes include alginate lyase, amylase, agarase, ι-carrageenase, cellulase, fucosidase, pectate lyase, xylosidase as well as various sulfatases. These results reveal that Echinicola strongylocentroti MEBiC08714T have potential to degrade seaweed polysaccharides. The genome sequence of Echinicola strongylocentroti MEBiC08714T provides the fundamental genomic information for future studies on novel depolymeration enzymes produced by marine bacteria for utilizing seaweed for biofuels and chemicals production.