제주도 홍해삼 (Stichopus japonicas)은 Raw264.7 macroropahges에서 MAPKs와 ROS 생성을 통한 염증 활동 억제

Abstract

Seacucmbers are used in formulation of traditional medicine in east asia for treatment of asthma, hypertension, and anemia. And it was known that it exhibits different activities depending on the habitat Therefore, in this study, we confirmed the anti-inflammatory effect of red seacucumber (Stichopus japonicus) from Jeju Island. We obtained a vacuum dried S. japonicas extracts (VDSEs) by treatment with MeOH or MeOH/DCM (2:1). Both extractions showed inhibition of NO production without toxicity. And VDSEs significantly inhibited prostaglandin E2 PGE2),proteinlevelofiNOSandCOX-2aswellaspro-inflammatorycytokinessuchasinterleukin-6(IL-6),IL-1β, and tumor necrosis factor-α (TNF-α) at 20 and 50 &micro g/ml. Further mechanistic studies showed that VDSEs suppressed the phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 also reduced reactive oxygen species (ROS) generation. Overall, our results demonstrated that S. japonicas extracts using different extraction methods presented anti-inflammatory activity by inhibiting MAPKs and ROS generation. Therefore, VDSEs can be used and applied as a useful anti-inflammatory agent.ed the anti-inflammatory effect of red seacucumber (Stichopus japonicus) from Jeju Island. We obtained a vacuum dried S. japonicas extracts (VDSEs) by treatment with MeOH or MeOH/DCM (2:1). Both extractions showed inhibition of NO production without toxicity. And VDSEs significantly inhibited prostaglandin E2 PGE2),proteinlevelofiNOSandCOX-2aswellaspro-inflammatorycytokinessuchasinterleukin-6(IL-6),IL-1β, and tumor necrosis factor-α (TNF-α) at 20 and 50 &micro g/ml. Further mechanistic studies showed that VDSEs suppressed the phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 also reduced reactive oxygen species (ROS) generation. Overall, our results demonstrated that S. japonicas extracts using different extraction methods presented anti-inflammatory activity by inhibiting MAPKs and ROS generation. Therefore, VDSEs can be used and applied as a useful anti-inflammatory agent.