Pseudofulvibacter gastropodicola sp nov., isolated from a marine conch and emended descriptions of the genus Pseudofulvibacter Yoon et al. 2013 and Pseudofulvibacter geojedonensis

Abstract

A Gram-stain-negative, aerobic, rod-shaped and non-motile marine bacterium, designated MEBiC08749(T), was isolated from a marine conch collected at Samcheok Port in the East Sea, Korea (also known as the Sea of Japan). 16S rRNA gene sequence analysis revealed that strain MEBiC08749(T) shared highest similarity with Pseudofulvibacter geojedonensis YCS-9(T) (94.9 %). Growth was observed at 5-31 degrees C (optimum, 25 degrees C), at pH 6.0-8.5 (optimum, pH 7.5) and with 0-6 % (w/v) NaCl (optimum, 2.5 %). The predominant cellular fatty acids were iso-C-15:0, iso-C-15:1 G, iso-C-15:0 3-OH, iso-C-17:0 3-OH, summed feature 3 (comprising C-16:1 omega 6c and/or C-16:1 omega 7c) and summed feature 9 (comprising 10-methyl C-16:0 and/or iso-C-17:1 omega 9c). The DNA G+C content was 30 mol%. The only respiratory quinone was menaquinone-6 (MK-6)., Phosphatidylethanolamine, two unidentified lipids and two unidentified aminolipids were detected as major polar lipids. Several phenotypic characteristics, such as production of acetoin, activity of cystine arylamidase, utilization of some carbohydrates, organic acids and amino acids, differentiated strain MEBiC08749T from P. geojedonensis KCTC 23884(T). On the basis of data from this polyphasic taxonomic study, strain MEBiC08749T should be classified as representing a novel species in the genus Pseudofulvibacter, for which the name Pseudofulvibacter gastropodicola sp. nov. is proposed. The type strain is MEBiC08749(T) (=KCCM 43046(T)=JCM 30369(T)). Emended descriptions of the genus Pseudofulvibacter Yoon et aL 2013 and of Pseudofulvibacter geojedonensis are also given.