Dimethyl sulfoxide reduction by a hyperhermophilic archaeon Thermococcus onnurineus NA1 via a cysteine-cystine redox shuttle SCIE SCOPUS KCI

Cited 5 time in WEB OF SCIENCE Cited 5 time in Scopus
Title
Dimethyl sulfoxide reduction by a hyperhermophilic archaeon Thermococcus onnurineus NA1 via a cysteine-cystine redox shuttle
Author(s)
Choi, Ae Ran; Kim, Min-Sik; Kang, Sung Gyun; Lee, Hyun Sook
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)
Publication Year
2016-01
Abstract
A variety of microbes grow by respiration with dimethyl sulfoxide (DMSO) as an electron acceptor, and several distinct DMSO respiratory systems, consisting of electron carriers and a terminal DMSO reductase, have been characterized. The heterotrophic growth of a hyperthermophilic archaeon Thermococcus onnurineus NA1 was enhanced by the addition of DMSO, but the archaeon was not capable of reducing DMSO to DMS directly using a DMSO reductase. Instead, the archaeon reduced DMSO via a cysteine-cystine redox shuttle through a mechanism whereby cystine is microbially reduced to cysteine, which is then reoxidized by DMSO reduction. A thioredoxin reductase-protein disulfide oxidoreductase redox couple was identified to have intracellular cystine-reducing activity, permitting recycle of cysteine. This study presents the first example of DMSO reduction via an electron shuffle. Several Thermococcales species also exhibited enhanced growth coupled with DMSO reduction, probably by disposing of excess reducing power rather than conserving energy.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2291
DOI
10.1007/s12275-016-5574-1
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.54, no.1, pp.31 - 38, 2016
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Subject
TRIMETHYLAMINE-N-OXIDE; COMPLETE GENOME SEQUENCE; SEA HYDROTHERMAL VENT; PROTEIN-DISULFIDE OXIDOREDUCTASE; ESCHERICHIA-COLI; METHIONINE SULFOXIDE; THIOREDOXIN REDUCTASE; ANAEROBIC RESPIRATION; ELECTRON-TRANSFER; DIMETHYLSULFOXIDE REDUCTION
Keywords
DMSO; Thermococcus onnurineus NA1; cysteine-cystine redox shuttle; thioredoxin reductase; protein disulfide oxidoreductase; extracellular electron mediator
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Related Researcher
Research Interests

Microbial biotechnology,Marine enzymes,미생물 활용,효소 개발

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