Apo-9′-fucoxanthinone suppresses inflammatory activity via MAPKs signaling pathway in LPS-stimulated Raw264.7 macrophages and zebrafish model
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Title
- Apo-9′-fucoxanthinone suppresses inflammatory activity via MAPKs signaling pathway in LPS-stimulated Raw264.7 macrophages and zebrafish model
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Author(s)
- 김은아; 김준성; 양현성; 강도형; 허수진
- KIOST Author(s)
- Kim, Eun A(김은아); Kim, Jun Seong(김준성); Yang, Hyun Sung(양현성); Kang, Do Hyung(강도형); Heo, Soo Jin(허수진)
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Alternative Author(s)
- 김은아; 김준성; 양현성; 강도형; 허수진
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Publication Year
- 2018-11-19
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Abstract
- In this study, we confirmed the anti-inflammatory effect of Apo-9-fucoxanthinone (AF) in in vitro RAW 264.7 cells and in vivo zebrafish model. In LPS-stimulated zebrafish, AF significantly decreased the production of reactive oxygen species (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of iNOS, suppressed COX-2 and an inflammatory cytokines IL-1β, TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264.7 cells. Further, AF suppressed COX-2, PGE2, and pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) at 25, 50 and 100 µ g/mL, respectively. Further mechanistic studies showed that AF suppressed the nuclear factor-kB (NF-kB) pathway and phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as ERK and JNK. According to the results, AF can be used and applied as a useful anti-inflammatory agent of nutraceutical or pharmaceutical. (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of iNOS, suppressed COX-2 and an inflammatory cytokines IL-1β, TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264.7 cells. Further, AF suppressed COX-2, PGE2, and pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) at 25, 50 and 100 µ g/mL, respectively. Further mechanistic studies showed that AF suppressed the nuclear factor-kB (NF-kB) pathway and phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as ERK and JNK. According to the results, AF can be used and applied as a useful anti-inflammatory agent of nutraceutical or pharmaceutical.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/22871
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Bibliographic Citation
- ISNFF, pp.95, 2018
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Publisher
- ISNFF
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Type
- Conference
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Language
- English
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