Apo-9′-fucoxanthinone suppresses inflammatory activity via MAPKs signaling pathway in LPS-stimulated Raw264.7 macrophages and zebrafish model

Title
Apo-9′-fucoxanthinone suppresses inflammatory activity via MAPKs signaling pathway in LPS-stimulated Raw264.7 macrophages and zebrafish model
Author(s)
김은아; 김준성; 양현성; 강도형; 허수진
KIOST Author(s)
Kim, Eun A(김은아)Kim, Jun Seong(김준성)Yang, Hyun Sung(양현성)Kang, Do Hyung(강도형)Heo, Soo Jin(허수진)
Alternative Author(s)
김은아; 김준성; 양현성; 강도형; 허수진
Publication Year
2018-11-19
Abstract
In this study, we confirmed the anti-inflammatory effect of Apo-9-fucoxanthinone (AF) in in vitro RAW 264.7 cells and in vivo zebrafish model. In LPS-stimulated zebrafish, AF significantly decreased the production of reactive oxygen species (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of iNOS, suppressed COX-2 and an inflammatory cytokines IL-1β, TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264.7 cells. Further, AF suppressed COX-2, PGE2, and pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) at 25, 50 and 100 µ g/mL, respectively. Further mechanistic studies showed that AF suppressed the nuclear factor-kB (NF-kB) pathway and phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as ERK and JNK. According to the results, AF can be used and applied as a useful anti-inflammatory agent of nutraceutical or pharmaceutical. (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of iNOS, suppressed COX-2 and an inflammatory cytokines IL-1β, TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264.7 cells. Further, AF suppressed COX-2, PGE2, and pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) at 25, 50 and 100 µ g/mL, respectively. Further mechanistic studies showed that AF suppressed the nuclear factor-kB (NF-kB) pathway and phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as ERK and JNK. According to the results, AF can be used and applied as a useful anti-inflammatory agent of nutraceutical or pharmaceutical.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/22871
Bibliographic Citation
ISNFF, pp.95, 2018
Publisher
ISNFF
Type
Conference
Language
English
Publisher
ISNFF
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