Saringosterol acetate는 zebrafish 모델에서 간세포 암종 억제

Abstract

Hizikia fusiforme, an edible brown alga widely consumed in Korea, Japan, and China has been found to possess a number of potential biological functionalities. The present study assessed the potent anti-cancer effects of saringostrol acetate (SSA) isolated from the H. fusiforme towards the inhibitory effects on hepatocellular carcinoma in Hep3B cell. SSA markedly inhibited cancer cell growth, attenuating the population of cells in sub-G1 through PI3K/Akt/mTOR pathway. Furtherstudies elaborate the therapeutic effects of SSA against diethylnitrosamin (DEN)-stimulated cancer in zebrafish model. DEN exposure caused a marked increase in reactive oxygen species (ROS) production, cell death, and expression of transforming growth factor beta (TGFβ) and metalloprotease 2 (MMP2) in zebrafish. However, treatment with SSA significantly downregulated DEN-induced ROS, cell death, and TGFβ and MMP2 expressions. These results support the potential anticancer effects of SSA and highlight its potential applications related to nutraceuticals or functional food to reduce the carcinogenic effect. (SSA) isolated from the H. fusiforme towards the inhibitory effects on hepatocellular carcinoma in Hep3B cell. SSA markedly inhibited cancer cell growth, attenuating the population of cells in sub-G1 through PI3K/Akt/mTOR pathway. Furtherstudies elaborate the therapeutic effects of SSA against diethylnitrosamin (DEN)-stimulated cancer in zebrafish model. DEN exposure caused a marked increase in reactive oxygen species (ROS) production, cell death, and expression of transforming growth factor beta (TGFβ) and metalloprotease 2 (MMP2) in zebrafish. However, treatment with SSA significantly downregulated DEN-induced ROS, cell death, and TGFβ and MMP2 expressions. These results support the potential anticancer effects of SSA and highlight its potential applications related to nutraceuticals or functional food to reduce the carcinogenic effect.