A Codon-Optimized Nucleic Acid Hydrolyzing Single-Chain Antibody Confers Resistance to Chrysanthemums Against Chrysanthemum Stunt Viroid Infection SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Tran, Dung Thi | - |
dc.contributor.author | Cho, Seungchan | - |
dc.contributor.author | Hoang, Phuong Mai | - |
dc.contributor.author | Kim, Jaedeok | - |
dc.contributor.author | Kil, Eui-Joon | - |
dc.contributor.author | Lee, Taek-Kyun | - |
dc.contributor.author | Rhee, Yong | - |
dc.contributor.author | Lee, Sukchan | - |
dc.date.accessioned | 2020-04-20T02:55:16Z | - |
dc.date.available | 2020-04-20T02:55:16Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.issn | 0735-9640 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2282 | - |
dc.description.abstract | Chrysanthemum stunt viroid (CSVd), the smallest plant pathogen known to infect chrysanthemums, is a single-stranded circular RNA viroid that induces stunting that results in an overall height reduction of 30-50 % in mature plants. A catalytic single-chain variable antibody, 3D8 scFv, which exhibits intrinsic DNase and RNase activities, was expressed in chrysanthemums to generate transgenic plant resistance to CSVd infection. Moreover, a codon-optimized version of the 3D8 scFv gene for chrysanthemums was also transformed into plants; these codon-optimized transgenic chrysanthemums expressed twice as much 3D8 scFv and displayed 60 % more resistance to CSVd infection, compared with transgenic chrysanthemums harboring the original 3D8 scFv gene. CSVd challenge experiments with codon-optimized and original 3D8 scFv-transgenic chrysanthemums showed that CSVd in newly produced leaves of both codon-optimized and original 3D8 scFv-transgenic plants was not detected by RT-PCR. This is the first report describing the development of a CSVd-resistant chrysanthemum harboring a catalytic single-chain antibody, 3D8 scFv, which has intrinsic RNase activity. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | TRANSGENIC PLANTS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | VIRUS | - |
dc.subject | SEQUENCE | - |
dc.subject | DNA | - |
dc.subject | RIBONUCLEASE | - |
dc.subject | STRATEGIES | - |
dc.subject | TOBACCO | - |
dc.subject | CELLS | - |
dc.subject | SCFV | - |
dc.title | A Codon-Optimized Nucleic Acid Hydrolyzing Single-Chain Antibody Confers Resistance to Chrysanthemums Against Chrysanthemum Stunt Viroid Infection | - |
dc.type | Article | - |
dc.citation.endPage | 232 | - |
dc.citation.startPage | 221 | - |
dc.citation.title | PLANT MOLECULAR BIOLOGY REPORTER | - |
dc.citation.volume | 34 | - |
dc.citation.number | 1 | - |
dc.contributor.alternativeName | 이택견 | - |
dc.identifier.bibliographicCitation | PLANT MOLECULAR BIOLOGY REPORTER, v.34, no.1, pp.221 - 232 | - |
dc.identifier.doi | 10.1007/s11105-015-0915-5 | - |
dc.identifier.scopusid | 2-s2.0-84955377609 | - |
dc.identifier.wosid | 000372924200018 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | TRANSGENIC PLANTS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | RIBONUCLEASE | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | TOBACCO | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | SCFV | - |
dc.subject.keywordAuthor | 3D8 scFv | - |
dc.subject.keywordAuthor | Agrobacterium-mediated transformation | - |
dc.subject.keywordAuthor | Chrysanthemum | - |
dc.subject.keywordAuthor | Chrysanthemum stunt viroid (CSVd) | - |
dc.subject.keywordAuthor | Codon optimization | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Plant Sciences | - |