A Codon-Optimized Nucleic Acid Hydrolyzing Single-Chain Antibody Confers Resistance to Chrysanthemums Against Chrysanthemum Stunt Viroid Infection SCIE SCOPUS

Cited 8 time in WEB OF SCIENCE Cited 9 time in Scopus
Title
A Codon-Optimized Nucleic Acid Hydrolyzing Single-Chain Antibody Confers Resistance to Chrysanthemums Against Chrysanthemum Stunt Viroid Infection
Author(s)
Tran, Dung Thi; Cho, Seungchan; Hoang, Phuong Mai; Kim, Jaedeok; Kil, Eui-Joon; Lee, Taek-Kyun; Rhee, Yong; Lee, Sukchan
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2016-02
Abstract
Chrysanthemum stunt viroid (CSVd), the smallest plant pathogen known to infect chrysanthemums, is a single-stranded circular RNA viroid that induces stunting that results in an overall height reduction of 30-50 % in mature plants. A catalytic single-chain variable antibody, 3D8 scFv, which exhibits intrinsic DNase and RNase activities, was expressed in chrysanthemums to generate transgenic plant resistance to CSVd infection. Moreover, a codon-optimized version of the 3D8 scFv gene for chrysanthemums was also transformed into plants; these codon-optimized transgenic chrysanthemums expressed twice as much 3D8 scFv and displayed 60 % more resistance to CSVd infection, compared with transgenic chrysanthemums harboring the original 3D8 scFv gene. CSVd challenge experiments with codon-optimized and original 3D8 scFv-transgenic chrysanthemums showed that CSVd in newly produced leaves of both codon-optimized and original 3D8 scFv-transgenic plants was not detected by RT-PCR. This is the first report describing the development of a CSVd-resistant chrysanthemum harboring a catalytic single-chain antibody, 3D8 scFv, which has intrinsic RNase activity.
ISSN
0735-9640
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2282
DOI
10.1007/s11105-015-0915-5
Bibliographic Citation
PLANT MOLECULAR BIOLOGY REPORTER, v.34, no.1, pp.221 - 232, 2016
Publisher
SPRINGER
Subject
TRANSGENIC PLANTS; ESCHERICHIA-COLI; VIRUS; SEQUENCE; DNA; RIBONUCLEASE; STRATEGIES; TOBACCO; CELLS; SCFV
Keywords
3D8 scFv; Agrobacterium-mediated transformation; Chrysanthemum; Chrysanthemum stunt viroid (CSVd); Codon optimization
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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