5-bromoprotocatechualdehyde suppresses lung cancer growth through regulation of ROS and the AKT/MAPKs signaling pathway in human lung cancer cells

Abstract

Background : Early asymptomatic lung cancer has the highest mortality rate in cancer. Therefore, we investigated anti-cancer activity of 5-bromoprotocatechualdehyde (BPCA) from polysiphonia morrowii Harvey (P. morrowii) in lung cancer H460lung cancer cells. Methods : Dried P. morrowii powder was extracted thrice with 80% aqueous methanol at room temperature. The extract was separated using HPLC and the chemical structure was confirmed 1H and 13C NMR date. BPCA identified the anti-cancer activity by cell viability, apoptotic assay, ROS generation and protein expression. Result : BPCA showed inhibition of tumor cell growth and induced decreased ROS generation which is associated with the induction of apoptosis though mitogen-activated protein kinase (MAPK) pathway and AKT signaling in lung cancer cells. The combination of BPCA and hydrogen peroxide (H2O2), increased ROS production and apoptosis compare with non-treated group via regulation of AKTsignaling and JNK MAPKs pathway. Conclusions : The result of the present study demonstrated that BPCA induced lung cancer cell death through ROS-mediated phosphorylation of AKT/MAPK signaling. Therefore, BPCA could be a beneficial and useful for treating lung cancer