Ishophloroglucin A From Ishige okamurae Alleviates The High Fat Diet-induced Obesity Through Leptin Signaling Pathway On Peripheral Metabolism

Abstract

Background Leptin, well known as an appetite hormone, plays a role in fat metabolism on peripheral tissues including adipose, liver, and muscle tissues. In here, we investigated the anti-obesity effects of Ishophloroglucin A (IPA) from Ishige okamurae through leptin signaling pathway on peripheral metabolism of a high fat diet obese mice model. Methods C57BL/6J obese mice model were organized by feeding high fat diet for 6 weeks and IPA (2.5 mg/kg) was orally treated for 4 weeks. After finishing the experiment, the several serum biochemical parameters were measured using ELISA or test kit, and the white adipose, liver, and muscle tissues were observed using immunohistochemistry methods. Results As a result, IPA treatment reduced the body weight, the circulating leptin level, total cholesterol, triglyceride, GOT, and GPT levels increased by high fat diet. Also, IPA treatment activated various leptin signaling pathways including Janus kinase 2 (JAK2)-signal transducer and activator of transcription (STAT), mechanistic target of rapamycin (mTOR)-ribosomal S6 kinase (S6K), and SH2-containing protein tyrosine phosphatase 2 (SHP2)-extracellular signal-regulated kinases (ERK) in the peripheral tissues. Moreover, IPA treatment reduced the adipose tissue size, and alleviated the steatosis in liver and muscle tissues.