Detection of the Cochlodinium polykrikoides On the Site using Real-Time PCR Method

Abstract

Cochlodinium polykrikoides is one of the most harmful microalgae causing algal blooms. It is responsible for impacting to aquaculture and economics. For managing algal blooms, It is necessary that improving rapid detection method on the site. In the present study, C. polykrikoides was detected on the site by real-time PCR method during September 2019 algal blooms of the Korean southern costal. LSU gene was selected as a target for C. polykrikoide, and standard curves were produced for quantitative cell number analysis. At 2nd and 4th September, which is first research, Yeosu, especially station G2, G17 and G20, was detect the algal bloom. It spread to southeastern water, including Namhae and Tongyeong. Next research period, at 11th and 12th, C. polykrikoides bloomed in Tongyeong, which is station R1, R2, R3, R5. Between first and second research period, there was a typhoon, LINGLING. It may be considered that typhoon put C. polykrikoides into the costal. In addition, Detection C. polykrikoides method was applied on the site. The results correspond with performing experiments in vitro and it could contribute to the rapid monitoring of C. polykrikoides in the field.