Efficient detection of pathogen virus in sand dabs, Paralichthys olivaceus using loop-mediated isothermal amplification (LAMP) SCIE SCOPUS

DC Field Value Language
dc.contributor.author Hwang, Jinik -
dc.contributor.author Park, So Yun -
dc.contributor.author Suh, Sung-Suk -
dc.contributor.author Park, Mirye -
dc.contributor.author Lee, Sukchan -
dc.contributor.author Lee, Taek-Kyun -
dc.date.accessioned 2020-04-20T02:25:47Z -
dc.date.available 2020-04-20T02:25:47Z -
dc.date.created 2020-01-28 -
dc.date.issued 2016-08 -
dc.identifier.issn 0253-505X -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/2167 -
dc.description.abstract Viral hemorrhagic septicemia virus (VHSV) and marine birnavirus (MABV) are the causative pathogens for some of the most explosive epidemics of emerging viral diseases in many Asian countries, leading to huge economic losses in aquaculture. Rapid molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of pathogen infection. The loop-mediated isothermal amplification (LAMP) of DNA is currently one of the most commonly used molecular diagnostic tools, as it is simple, quick, and easy to amplify target DNA under isothermal conditions. In the present study, a novel and highly specific LAMP assay for the sensitive and rapid detection of VHSV and MABV infection in fish was developed. Using a set of synthesized primers matching a specific region of the genome, the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficient detection of VHSV and MABV infection in the wild fish, Paralichthys olivaceus. Our results demonstrate the simplicity and convenience of this method for the detection of viral infection in aquatic organisms. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER -
dc.subject NECROSIS VIRUS -
dc.subject RT-PCR -
dc.subject FISH -
dc.subject VHSV -
dc.subject RSIV -
dc.title Efficient detection of pathogen virus in sand dabs, Paralichthys olivaceus using loop-mediated isothermal amplification (LAMP) -
dc.type Article -
dc.citation.endPage 50 -
dc.citation.startPage 44 -
dc.citation.title ACTA OCEANOLOGICA SINICA -
dc.citation.volume 35 -
dc.citation.number 8 -
dc.identifier.bibliographicCitation ACTA OCEANOLOGICA SINICA, v.35, no.8, pp.44 - 50 -
dc.identifier.doi 10.1007/s13131-016-0889-7 -
dc.identifier.scopusid 2-s2.0-84981278182 -
dc.identifier.wosid 000381248900007 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus MARINE BIRNAVIRUS MABV -
dc.subject.keywordPlus SEA BREAM IRIDOVIRUS -
dc.subject.keywordPlus JAPANESE FLOUNDER -
dc.subject.keywordPlus NECROSIS VIRUS -
dc.subject.keywordPlus RT-PCR -
dc.subject.keywordPlus QUANTIFICATION -
dc.subject.keywordPlus FISH -
dc.subject.keywordPlus VHSV -
dc.subject.keywordPlus RSIV -
dc.subject.keywordAuthor viral hemorrhagic septicaemia virus (VHSV) -
dc.subject.keywordAuthor marine birnavirus (MABV) -
dc.subject.keywordAuthor polymerase chain reaction -
dc.subject.keywordAuthor loop-mediated isothermal amplification -
dc.relation.journalWebOfScienceCategory Oceanography -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Oceanography -
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South Sea Research Institute > Risk Assessment Research Center > 1. Journal Articles
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