Efficient detection of pathogen virus in sand dabs, Paralichthys olivaceus using loop-mediated isothermal amplification (LAMP) SCIE SCOPUS

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Title
Efficient detection of pathogen virus in sand dabs, Paralichthys olivaceus using loop-mediated isothermal amplification (LAMP)
Author(s)
Hwang, Jinik; Park, So Yun; Suh, Sung-Suk; Park, Mirye; Lee, Sukchan; Lee, Taek-Kyun
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2016-08
Abstract
Viral hemorrhagic septicemia virus (VHSV) and marine birnavirus (MABV) are the causative pathogens for some of the most explosive epidemics of emerging viral diseases in many Asian countries, leading to huge economic losses in aquaculture. Rapid molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of pathogen infection. The loop-mediated isothermal amplification (LAMP) of DNA is currently one of the most commonly used molecular diagnostic tools, as it is simple, quick, and easy to amplify target DNA under isothermal conditions. In the present study, a novel and highly specific LAMP assay for the sensitive and rapid detection of VHSV and MABV infection in fish was developed. Using a set of synthesized primers matching a specific region of the genome, the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficient detection of VHSV and MABV infection in the wild fish, Paralichthys olivaceus. Our results demonstrate the simplicity and convenience of this method for the detection of viral infection in aquatic organisms.
ISSN
0253-505X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2167
DOI
10.1007/s13131-016-0889-7
Bibliographic Citation
ACTA OCEANOLOGICA SINICA, v.35, no.8, pp.44 - 50, 2016
Publisher
SPRINGER
Subject
MARINE BIRNAVIRUS MABV; SEA BREAM IRIDOVIRUS; JAPANESE FLOUNDER; NECROSIS VIRUS; RT-PCR; QUANTIFICATION; FISH; VHSV; RSIV
Keywords
viral hemorrhagic septicaemia virus (VHSV); marine birnavirus (MABV); polymerase chain reaction; loop-mediated isothermal amplification
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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