Metagenomic analysis reveals the contribution of anaerobic methanotroph-1b in the oxidation of methane at the Ulleung Basin, East Sea of Korea SCIE SCOPUS KCI

Cited 3 time in WEB OF SCIENCE Cited 3 time in Scopus
Title
Metagenomic analysis reveals the contribution of anaerobic methanotroph-1b in the oxidation of methane at the Ulleung Basin, East Sea of Korea
Author(s)
Lee, Jin-Woo; Kwon, Kae Kyoung; Bahk, Jang-Jun; Lee, Dong-Hun; Lee, Hyun Sook; Kang, Sung Gyun; Lee, Jung-Hyun
KIOST Author(s)
Kwon, Kae Kyoung(권개경)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2016-12
Abstract
We have previously identified a sulfate methane transition zone (SMTZ) within the methane hydrate-bearing sediment in the Ulleung Basin, East Sea of Korea, and the presence of ANME-1b group in the sediment has been shown by phylogenetic analysis of a 16S rRNA gene. Herein, we describe taxonomic and functional profiling in the SMTZ sample by metagenomic analysis, comparing with that of surface sediment. Metagenomic sequences of 115 Mbp and 252 Mbp were obtained from SMTZ and surface sediments, respectively. The taxonomic profiling using BLASTX against the SEED within MG-RAST showed the prevalence of methanogens (19.1%), such as Methanosarcinales (12.0%) and Methanomicrobiales (4.1%) predominated within the SMTZ metagenome. A number of 185,200 SMTZ reads (38.9%) and 438,484 surface reads (62.5%) were assigned to functional categories, and methanogenesis-related reads were statistically significantly overrepresented in the SMTZ metagenome. However, the mapping analysis of metagenome reads to the reference genomes, most of the sequences of the SMTZ metagenome were mapped to ANME-1 draft genomes, rather than those of methanogens. Furthermore, the two copies of the methyl-coenzyme M reductase gene (mcrA) segments of the SMTZ metagenome were clustered with ANME-1b in the phylogenetic cluster. These results indicate that ANME-1b reads were miss-annotated to methanogens due to limitation of database. Many of key genes necessary for reverse methanogenesis were present in the SMTZ metagenome, except for N (5),N (10)-methenyl-H4MPT reductase (mer) and CoB-CoM heterodisulfide reductase subunits D and E (hdrDE). These data suggest that the ANME-1b represents the primary player the anaerobic methane oxidation in the SMTZ, of the methane hydrate-bearing sediment at the Ulleung Basin, East Sea of Korea.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2147
DOI
10.1007/s12275-016-6379-y
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.54, no.12, pp.814 - 822, 2016
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Subject
COLD SEEP SEDIMENTS; HETERODISULFIDE REDUCTASE; METHANOSARCINA-BARKERI; ARCHAEA; METHANOGENESIS; PURIFICATION; COMMUNITIES; CONSORTIUM; BACTERIA; NICKEL
Keywords
sulfate methane transition zone (SMTZ); anaerobic methanotroph-1b (ANME-1b); reverse methanogenesis pathway; metagenomics
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse