Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri vent field of the Central Indian Ocean ridge

Title
Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri vent field of the Central Indian Ocean ridge
Author(s)
임재규; 김윤재; 양정안; Teddy Namirimu; 양성현; 박미정; 권용민; 이현숙; 강성균; 이정현; 권개경
KIOST Author(s)
Yang, Jeongan(양정안)Namirimu, Teddy(Teddy Namirimu)Park, Mi Jeong(박미정)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)Kwon, Kae Kyoung(권개경)
Alternative Author(s)
임재규; 김윤재; 양정안; Teddy Namirimu; 양성현; 박미정; 이현숙; 강성균; 이정현; 권개경
Publication Year
2019-11-08
Abstract
A strictly anaerobic, dissimilatory Fe(III)-reducing hyperthermophilic archaeon, designated as strain IOH1T, was isolated from a new deep-sea hydrothermal vent (Onnuri Vent Field) area in the Central Indian Ocean ridge. Strain IOH1T showed> 99% 16S rRNA gene sequence similarity with Thermococcus celericrescens TS2T (99.4%) and T. siculi DSM 12349T (99.2%). Additional three species T. barossii SHCK-94T (99.0%), T. celer Vu13T (98.8%), and T. piezophilus (98.6%) showed >98.6%of 16S rRNA gene sequence similarity, however, the maximum OrthoANI value is 89.8% for the genome of T. celericrescens TS2T. Strain IOH1T cells are coccoid, 1.2-1.8 mm in diameter, and motile by flagella. Growth was at 70-82℃, pH 5.4-8.0 (optimum pH 6.0) with 2-4% NaCl. Growth of strain IOH1T was enhanced by starch, pyruvate, D(+)-maltose and maltodextrin as a carbon sources, and elemental sulfur as an electron acceptor clearly different from those of related species T. celecrescens DSM 17994T and T. siculi DSM 12349T. Strain IOH1T, T. celercrescence DSM 17994T, and T. siculi DSM 12349T reduced soluble Fe(III)-citrate present in the medium, whereas the amount of total cellular proteins increased with the concomitant accumulation of Fe(II). We determined a circular chromosome of 2,234 kb with an extra-chromosomal archaeal plasmid, pTI1, of 7.7 kb and predicted 2,425 genes. The DNA G + C content was 54.9 mol%. Based on physiological properties, p
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/21075
Bibliographic Citation
한국해양바이오학회, pp.1, 2019
Publisher
한국해양바이오학회
Type
Conference
Language
English
Publisher
한국해양바이오학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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