Anti-inflammatory Activity on LPS-stimulated in vitro RAW 264.7 Cells and in vivo Zebrafish of Heterosigma akshiwo
KCI
-
Title
- Anti-inflammatory Activity on LPS-stimulated in vitro RAW 264.7 Cells and in vivo Zebrafish of Heterosigma akshiwo
-
Author(s)
- 김준성; 최윤경; 이지혁; 김서영; 김현수; 전유진; 허수진
- KIOST Author(s)
- Kim, Jun Seong(김준성); Heo, Soo Jin(허수진)
-
Alternative Author(s)
- 김준성; 최윤경; 허수진
-
Publication Year
- 2017-09
-
Abstract
- Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the class Raphidophyceae, causes extensive damage to all marine ecosystems. It is essential to reduce the damage to marine ecosystems for them to be used as a resource. In this study, we used organic solvent fractionation to obtain an ethyl acetate–methanol extract from H. akashiwo (HAEM80) and then evaluated its anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and a zebrafish model. HAME80 markedly inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2). It also down-regulated the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and decreased the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. HAME80 reduced yolk edema and improved the survival rate of LPS-stimulated zebrafish embryos; in addition, the extract significantly reduced the production of ROS and NO and attenuated cell death in this model. Gas chromatography–mass spectrometry (GC-MS) of the extract was used to confirm the identity of peaks 1-20. Taken together, our data suggest that H. akashiwo is a beneficial anti-inflammatory agent.
-
ISSN
- 1229-4160
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2058
-
Bibliographic Citation
- Journal of Chitin and Chitosan, v.22, no.3, pp.185 - 193, 2017
-
Publisher
- 한국키틴키토산학회
-
Keywords
- Heterosigma akashiwo; Anti-inflammatoin; Zebrafish; GC-MS; RAW 264.7 cell
-
Type
- Article
-
Language
- English
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.