Anti-inflammatory Activity on LPS-stimulated in vitro RAW 264.7 Cells and in vivo Zebrafish of Heterosigma akshiwo KCI

Title
Anti-inflammatory Activity on LPS-stimulated in vitro RAW 264.7 Cells and in vivo Zebrafish of Heterosigma akshiwo
Author(s)
김준성; 최윤경; 이지혁; 김서영; 김현수; 전유진; 허수진
KIOST Author(s)
Kim, Junseong(김준성)Heo, Soo Jin(허수진)
Publication Year
2017-09
Abstract
Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the class Raphidophyceae, causes extensive damage to all marine ecosystems. It is essential to reduce the damage to marine ecosystems for them to be used as a resource. In this study, we used organic solvent fractionation to obtain an ethyl acetate–methanol extract from H. akashiwo (HAEM80) and then evaluated its anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and a zebrafish model. HAME80 markedly inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2). It also down-regulated the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and decreased the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. HAME80 reduced yolk edema and improved the survival rate of LPS-stimulated zebrafish embryos; in addition, the extract significantly reduced the production of ROS and NO and attenuated cell death in this model. Gas chromatography–mass spectrometry (GC-MS) of the extract was used to confirm the identity of peaks 1-20. Taken together, our data suggest that H. akashiwo is a beneficial anti-inflammatory agent.
ISSN
1229-4160
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2058
Bibliographic Citation
Journal of Chitin and Chitosan, v.22, no.3, pp.185 - 193, 2017
Publisher
한국키틴키토산학회
Keywords
Heterosigma akashiwo; Anti-inflammatoin; Zebrafish; GC-MS; RAW 264.7 cell
Type
Article
Language
English
Publisher
한국키틴키토산학회
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

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