Studies on the adaptive changes of a hyperthermophilic archaeon Thermococcus onnurineus NA1 under long-term selection on carbon monoxide

Title
Studies on the adaptive changes of a hyperthermophilic archaeon Thermococcus onnurineus NA1 under long-term selection on carbon monoxide
Author(s)
강성균; 이성혁; 김민식; 이재학; 이정현; 이현숙
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Seong Hyuk(이성혁)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)
Publication Year
2015-12-16
Abstract
Adaptive laboratory evolution is one of engineering approaches to produce new and fit phenotypes by long-term selection under a defined condition. To understand the molecular mechanisms driving adaptive phenotypic change, systems-level analysis such as comparative whole-genome sequencing and polyomic analysis has been performed. In this study, we evolved a hyperthermophilic archaeon Thermococcus onnurineus NA1 through serial transfers of a culture to fresh medium with carbon monoxide (CO) over 100 times. The changes in the phenotype, genome sequence and transcriptome were analyzed. The growth rate, CO consumption rate and H2 production rate of the strain gradually increased with transfer time. The genome of evolved strains was sequenced and several mutations were identified in comparison with a parental strain. The gene expression pattern obtained by transcriptome analysis was consistent with the phenotypic changes, and some genes involved in CO utilization such as carbon monoxide dehydrogenase, hydrogenase, Na+/H+-antiporter and ATP synthetase were significantly upregulated along with the adaption. This study demonstrates for the first time that CO-utilizing ability of microbes can be improved by adaptive evolutionary approach.ysis such as comparative whole-genome sequencing and polyomic analysis has been performed. In this study, we evolved a hyperthermophilic archaeon Thermococcus onnurineus NA1 through serial transfers of a culture to fresh medium with carbon monoxide (CO) over 100 times. The changes in the phenotype, genome sequence and transcriptome were analyzed. The growth rate, CO consumption rate and H2 production rate of the strain gradually increased with transfer time. The genome of evolved strains was sequenced and several mutations were identified in comparison with a parental strain. The gene expression pattern obtained by transcriptome analysis was consistent with the phenotypic changes, and some genes involved in CO utilization such as carbon monoxide dehydrogenase, hydrogenase, Na+/H+-antiporter and ATP synthetase were significantly upregulated along with the adaption. This study demonstrates for the first time that CO-utilizing ability of microbes can be improved by adaptive evolutionary approach.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/20513
Bibliographic Citation
The 2015 International Chemical Congress of Pacific Basin Societies (PAC CHEM™), pp.217, 2015
Publisher
American Chemical Society
Type
Conference
Language
English
Publisher
American Chemical Society
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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