A rapid and sensitive enzymatic assay for 2,3-butanediol SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Lee, Gyu Bi | - |
dc.contributor.author | Kim, Yun Jae | - |
dc.contributor.author | Lim, Jae Kyu | - |
dc.contributor.author | Kim, Tae Wan | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.contributor.author | Lee, Jung-Hyun | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.date.accessioned | 2020-04-20T02:05:01Z | - |
dc.date.available | 2020-04-20T02:05:01Z | - |
dc.date.created | 2020-02-04 | - |
dc.date.issued | 2019-05 | - |
dc.identifier.issn | 2190-572X | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2049 | - |
dc.description.abstract | In this study, we developed a rapid and sensitive enzymatic assay for 2,3-butanediol (2,3-BDO) detection. The concentration of 2,3-BDO was determined by measuring the reduction of NADP(+) using Clostridium ljungdahlii 2,3-butanediol dehydrogenase (CL-Bdh). The enzymatic assay could detect as low as 0.01 mM of 2,3-BDO, while the high-performance liquid chromatography (HPLC) method required a much higher concentration than 0.15 mM. Gratifyingly, the developed method was 15 times more sensitive than the HPLC method. When the enzymatic assay was applied to high-throughput screening, the enzymatic assay detected 14 positive samples out of 23 tested, as compared to 8 by the HPLC method. These results suggest that the enzymatic assay is an effective screening method for the detection of 2,3-BDO-producing microbes in a microtiter plate-based format. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.subject | ACETOIN | - |
dc.title | A rapid and sensitive enzymatic assay for 2,3-butanediol | - |
dc.type | Article | - |
dc.citation.title | 3 BIOTECH | - |
dc.citation.volume | 9 | - |
dc.citation.number | 5 | - |
dc.contributor.alternativeName | 이규비 | - |
dc.contributor.alternativeName | 김윤재 | - |
dc.contributor.alternativeName | 임재규 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.identifier.bibliographicCitation | 3 BIOTECH, v.9, no.5 | - |
dc.identifier.doi | 10.1007/s13205-019-1705-9 | - |
dc.identifier.scopusid | 2-s2.0-85064221545 | - |
dc.identifier.wosid | 000464107500002 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ACETOIN | - |
dc.subject.keywordAuthor | 2,3-Butanediol | - |
dc.subject.keywordAuthor | Enzymatic assay | - |
dc.subject.keywordAuthor | 2,3-Butanediol dehydrogenase | - |
dc.subject.keywordAuthor | High-throughput screening | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |