A rapid and sensitive enzymatic assay for 2,3-butanediol SCIE SCOPUS

Cited 1 time in WEB OF SCIENCE Cited 1 time in Scopus
Title
A rapid and sensitive enzymatic assay for 2,3-butanediol
Author(s)
Lee, Gyu Bi; Kim, Yun Jae; Lim, Jae Kyu; Kim, Tae Wan; Kang, Sung Gyun; Lee, Jung-Hyun; Lee, Hyun Sook
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)
Publication Year
2019-05
Abstract
In this study, we developed a rapid and sensitive enzymatic assay for 2,3-butanediol (2,3-BDO) detection. The concentration of 2,3-BDO was determined by measuring the reduction of NADP(+) using Clostridium ljungdahlii 2,3-butanediol dehydrogenase (CL-Bdh). The enzymatic assay could detect as low as 0.01 mM of 2,3-BDO, while the high-performance liquid chromatography (HPLC) method required a much higher concentration than 0.15 mM. Gratifyingly, the developed method was 15 times more sensitive than the HPLC method. When the enzymatic assay was applied to high-throughput screening, the enzymatic assay detected 14 positive samples out of 23 tested, as compared to 8 by the HPLC method. These results suggest that the enzymatic assay is an effective screening method for the detection of 2,3-BDO-producing microbes in a microtiter plate-based format.
ISSN
2190-572X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2049
DOI
10.1007/s13205-019-1705-9
Bibliographic Citation
3 BIOTECH, v.9, no.5, 2019
Publisher
SPRINGER HEIDELBERG
Subject
ACETOIN
Keywords
2,3-Butanediol; Enzymatic assay; 2,3-Butanediol dehydrogenase; High-throughput screening
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER HEIDELBERG
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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