A rapid and sensitive enzymatic assay for 2,3-butanediol
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Title
- A rapid and sensitive enzymatic assay for 2,3-butanediol
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Author(s)
- Lee, Gyu Bi; Kim, Yun Jae; Lim, Jae Kyu; Kim, Tae Wan; Kang, Sung Gyun; Lee, Jung-Hyun; Lee, Hyun Sook
- KIOST Author(s)
- Kim, Yun Jae(김윤재); Lim, Jae Kyu(임재규); Kang, Sung Gyun(강성균); Lee, Jung Hyun(이정현); Lee, Hyun Sook(이현숙)
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Alternative Author(s)
- 이규비; 김윤재; 임재규; 강성균; 이정현; 이현숙
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Publication Year
- 2019-05
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Abstract
- In this study, we developed a rapid and sensitive enzymatic assay for 2,3-butanediol (2,3-BDO) detection. The concentration of 2,3-BDO was determined by measuring the reduction of NADP(+) using Clostridium ljungdahlii 2,3-butanediol dehydrogenase (CL-Bdh). The enzymatic assay could detect as low as 0.01 mM of 2,3-BDO, while the high-performance liquid chromatography (HPLC) method required a much higher concentration than 0.15 mM. Gratifyingly, the developed method was 15 times more sensitive than the HPLC method. When the enzymatic assay was applied to high-throughput screening, the enzymatic assay detected 14 positive samples out of 23 tested, as compared to 8 by the HPLC method. These results suggest that the enzymatic assay is an effective screening method for the detection of 2,3-BDO-producing microbes in a microtiter plate-based format.
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ISSN
- 2190-572X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2049
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DOI
- 10.1007/s13205-019-1705-9
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Bibliographic Citation
- 3 BIOTECH, v.9, no.5, 2019
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Publisher
- SPRINGER HEIDELBERG
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Subject
- ACETOIN
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Keywords
- 2,3-Butanediol; Enzymatic assay; 2,3-Butanediol dehydrogenase; High-throughput screening
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Type
- Article
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Language
- English
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Document Type
- Article
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