박테리아에서 유래한 아키아와 같은 특징을 가진 새로운 샤페로닌의 구조분석

Title
박테리아에서 유래한 아키아와 같은 특징을 가진 새로운 샤페로닌의 구조분석
Alternative Title
Structural Analysis of Novel Archaeal-like Chaperonin from a Thermphilic bacterium
Author(s)
안영준; 차선신
KIOST Author(s)
An, Young Jun(안영준)
Alternative Author(s)
안영준
Publication Year
2018-06-27
Abstract
The chaperonins (CPNs) are megadalton sized hollow complexes with two cavities that open and close to encapsulate non-native proteins. CPNs are assigned to two sequence-related groups that have distinct allosteric mechanisms. In Group I CPNs a detachable co-chaperone, GroES, closes the chambers whereas in Group II a built-in lid closes the chambers. Group I CPNs have a bacterial ancestry, whereas Group II CPNs are archaeal in origin. Here we describe open and closed crystal structures representing a new phylogenetic branch of CPNs. These Group III CPNs are divergent in sequence and structure from extant CPNs, but are closed by a built-in lid like Group II CPNs. A nucleotide-sensing loop, present in both Group I and Group II CPNs, is notably absent. We identified inter-ring pivot joints that articulate during ring closure. These Group III CPNs likely represent a relic from the ancestral CPN that formed distinct bacterial and archaeal branches.s a detachable co-chaperone, GroES, closes the chambers whereas in Group II a built-in lid closes the chambers. Group I CPNs have a bacterial ancestry, whereas Group II CPNs are archaeal in origin. Here we describe open and closed crystal structures representing a new phylogenetic branch of CPNs. These Group III CPNs are divergent in sequence and structure from extant CPNs, but are closed by a built-in lid like Group II CPNs. A nucleotide-sensing loop, present in both Group I and Group II CPNs, is notably absent. We identified inter-ring pivot joints that articulate during ring closure. These Group III CPNs likely represent a relic from the ancestral CPN that formed distinct bacterial and archaeal branches.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/20446
Bibliographic Citation
2018 International Conference of the Korean Society for Structural Biology, pp.15, 2018
Publisher
한국구조생물학회
Type
Conference
Language
Korean
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