박테리아에서 유래한 아키아와 같은 특징을 가진 새로운 샤페로닌의 구조분석

Title
박테리아에서 유래한 아키아와 같은 특징을 가진 새로운 샤페로닌의 구조분석
Alternative Title
Structural Analysis of Novel Archaeal-like Chaperonin from a Thermphilic bacterium
Author(s)
안영준; 차선신
KIOST Author(s)
Young, Jun An(안영준)
Publication Year
2018-06-27
Abstract
The chaperonins (CPNs) are megadalton sized hollow complexes with two cavities that open and close to encapsulate non-native proteins. CPNs are assigned to two sequence-related groups that have distinct allosteric mechanisms. In Group I CPNs a detachable co-chaperone, GroES, closes the chambers whereas in Group II a built-in lid closes the chambers. Group I CPNs have a bacterial ancestry, whereas Group II CPNs are archaeal in origin. Here we describe open and closed crystal structures representing a new phylogenetic branch of CPNs. These Group III CPNs are divergent in sequence and structure from extant CPNs, but are closed by a built-in lid like Group II CPNs. A nucleotide-sensing loop, present in both Group I and Group II CPNs, is notably absent. We identified inter-ring pivot joints that articulate during ring closure. These Group III CPNs likely represent a relic from the ancestral CPN that formed distinct bacterial and archaeal branches.s a detachable co-chaperone, GroES, closes the chambers whereas in Group II a built-in lid closes the chambers. Group I CPNs have a bacterial ancestry, whereas Group II CPNs are archaeal in origin. Here we describe open and closed crystal structures representing a new phylogenetic branch of CPNs. These Group III CPNs are divergent in sequence and structure from extant CPNs, but are closed by a built-in lid like Group II CPNs. A nucleotide-sensing loop, present in both Group I and Group II CPNs, is notably absent. We identified inter-ring pivot joints that articulate during ring closure. These Group III CPNs likely represent a relic from the ancestral CPN that formed distinct bacterial and archaeal branches.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/20446
Bibliographic Citation
2018 International Conference of the Korean Society for Structural Biology, pp.15, 2018
Publisher
한국구조생물학회
Type
Conference
Language
Korean
Publisher
한국구조생물학회
Related Researcher
Research Interests

Structural biology,Protein Engineering,구조생물학,단백질 공학

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