유기주석화합물에 단기간 노출시킨 넙치 간장 약물대사효소의 in vivo 및 in vitro 반응 KCI OTHER

Title
유기주석화합물에 단기간 노출시킨 넙치 간장 약물대사효소의 in vivo 및 in vitro 반응
Alternative Title
In viro and In vivo Responses of MFO systems in Olive Flounder (Paralichthys olivaceus) Exposed to TBT and TPT for Short-term Period
Author(s)
전중균; 이지선; 전미정; 심원준; 임한규
KIOST Author(s)
Shim, Won Joon(심원준)
Publication Year
2004-03
Abstract
Cytochrome P450 (CYP) contents and 7 -ethoxyresorufin -O -deethylase(EROD) activity were determined in hepatic microsome of olive flounder ( Paralichthysolivaceus ) exposed to tributyltin chloride (TBTC), tributyltin oxide (TBTO), andtriphenyltin chloride (TPTC). In addition, effects of in vivo (intraperitoneal injection of7.5mg kg-1 BW) exposure of flounder to TPTC on CYP, NADPH cytochrome c reductase,NADH cytochrome b5 reductase and EROD levels were measured. In in vitro exposureof hepatic microsome to organotins, TBTC, TBTO and TPTC reduced CYP contents andinhibited EROD activity. The TPTC was the strongest inhibitor, which is followed byTBTO and TBTC. The degree of inhibition, especially EROD acitivity, depended on theexposure duration. In addition, all the target enzymes in flounder were inhibited byTPTC with the in vivo exposure to TPTC. As EROD activity was the most sensitive tothe inhibitions and demonstrated good reproducibility of the results, it could be usedas a helpful tool for monitor effects of organotin compounds on mixed funcitonoxygenase system in marine fish.
ISSN
1226-9999
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2013
Bibliographic Citation
환경생물, v.22, no.1, pp.177 - 183, 2004
Publisher
한국환경생물학회
Keywords
flounder; in vivo; TBT; TPT; cytochrome P450; EROD; NADPH cytochromec reductase; NADH cytochrome b5 reductase
Type
Article
Publisher
한국환경생물학회
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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