유기주석화합물에 단기간 노출시킨 넙치 간장 약물대사효소의 in vivo 및 in vitro 반응

Abstract

Cytochrome P450 (CYP) contents and 7 -ethoxyresorufin -O -deethylase(EROD) activity were determined in hepatic microsome of olive flounder ( Paralichthysolivaceus ) exposed to tributyltin chloride (TBTC), tributyltin oxide (TBTO), andtriphenyltin chloride (TPTC). In addition, effects of in vivo (intraperitoneal injection of7.5mg kg-1 BW) exposure of flounder to TPTC on CYP, NADPH cytochrome c reductase,NADH cytochrome b5 reductase and EROD levels were measured. In in vitro exposureof hepatic microsome to organotins, TBTC, TBTO and TPTC reduced CYP contents andinhibited EROD activity. The TPTC was the strongest inhibitor, which is followed byTBTO and TBTC. The degree of inhibition, especially EROD acitivity, depended on theexposure duration. In addition, all the target enzymes in flounder were inhibited byTPTC with the in vivo exposure to TPTC. As EROD activity was the most sensitive tothe inhibitions and demonstrated good reproducibility of the results, it could be usedas a helpful tool for monitor effects of organotin compounds on mixed funcitonoxygenase system in marine fish.