개선된 thin-layer chromatography를 이용한 바이오디젤 중의 글리세롤 정량분석 KCI

DC Field Value Language
dc.contributor.author 이상은 -
dc.contributor.author 최우석 -
dc.contributor.author 강도형 -
dc.contributor.author 이현용 -
dc.contributor.author 정경환 -
dc.date.accessioned 2020-04-19T23:40:52Z -
dc.date.available 2020-04-19T23:40:52Z -
dc.date.created 2020-02-10 -
dc.date.issued 2013-04 -
dc.identifier.issn 1225-9918 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/1680 -
dc.description.abstract We analyzed glycerol using thin-layer chromatography (TLC) and compared the separation resolution of some mobile phases. When acetonitrile:distilled water (85:15 v/v) was used as a mobile phase, the band of glycerol on the TLC was more distinctly and rapidly separated. Using TLC analysis, we prepared a calibration curve for the glycerol concentration vs. the area of the glycerol band in which the glycerol concentration of the x-axis was converted into a log-scale ranging from 3.0 to 0.0625 (%, w/v). Based on this calibration curve, the residual glycerol concentration (0.2 [%, w/v]) in biodiesel was determined successfully using TLC analysis. When the results of the TLC analysis were compared with those of a chemical and enzymatic assay, the results were fairly similar. We conclude that TLC without additional analytical instruments can be used as an alternative method for the quantitative analysis of the concentration of glycerol in biodiesel. Biodiesel 중의 glycerol을 분석하기 위하여 TLC를 이용하여 몇 가지 보고된 이동상에서 glycerol을 분석하였다. 그 중에서 acetonitrile : distilled water (85:15, v/v)를 이동상으로 하여 TLC를 수행하였을 경우에 짧은 시간에 명확한 glycerol band를 확인할 수 있었다. X축의 glycerol 농도를 log scale로 하고, TLC의 glycerol 이미지 면적을 Y축으로 하여 3.0-0.0625 (%, w/v)의 glycerol 농도범위에서 표준곡선을 작성할 수 있었으며, 이를 이용하여 0.2 (%, w/v)의 glycerol을 포함하는 biodiesel 시료에서 유의성 있게 glycerol을 정량분석 할 수 있음을 확인하였다. 이러한 결과는 chemical assay, enzymatic assay를 이용한 glycerol 분석과 비교하여 매우 유사한 결과이며, TLC를 이용한 biodiesel 중의 glycerol 정량법은 특별한 분석기기를 사용할지 않아도 되는 편리하고 간편한 방법으로 생각되어진다. -
dc.description.uri 2 -
dc.language Korean -
dc.publisher 한국생명과학회 -
dc.title 개선된 thin-layer chromatography를 이용한 바이오디젤 중의 글리세롤 정량분석 -
dc.title.alternative Improvement in Thin-layer Chromatography in a Quantitative Assay of Glycerol in Biodiesel -
dc.type Article -
dc.citation.endPage 541 -
dc.citation.startPage 537 -
dc.citation.title 생명과학회지 -
dc.citation.volume 24 -
dc.citation.number 4 -
dc.identifier.bibliographicCitation 생명과학회지, v.24, no.4, pp.537 - 541 -
dc.identifier.doi 10.5352/JLS.2013.23.4.537 -
dc.identifier.kciid ART001767457 -
dc.description.journalClass 2 -
dc.description.isOpenAccess N -
dc.subject.keywordAuthor Biodiesel -
dc.subject.keywordAuthor glycerol -
dc.subject.keywordAuthor thin-layer chromatography -
dc.subject.keywordAuthor quantitative analysis -
dc.subject.keywordAuthor pre-screening -
dc.description.journalRegisteredClass kci -
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Jeju Research Institute > Jeju Marine Research Center > 1. Journal Articles
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