Transcriptomic change as evidence for cadmium-induced endocrine disruption in marine fish model of medaka, Oryzias javanicus SCIE SCOPUS KCI

Cited 19 time in WEB OF SCIENCE Cited 25 time in Scopus
Title
Transcriptomic change as evidence for cadmium-induced endocrine disruption in marine fish model of medaka, Oryzias javanicus
Author(s)
Kim, Youn-Jung; Lee, Nayoung; Woo, Seonock; Ryu, Jae-Chun; Yum, Seungshic
KIOST Author(s)
Lee, Na Young(이나영)Woo, Seon Ock(우선옥)Yum, Seung Shic(염승식)
Alternative Author(s)
이나영; 우선옥; 염승식
Publication Year
2016-12-31
Abstract
We evaluated cadmium (Cd)-induced acute toxicity in Oryzias javanicus (marine medaka or Javanese ricefish) and gathered transcriptomic evidence for the Cd-induced endocrine-disrupting effect. The median lethal concentrations for the fish were determined to be 44.25 and 27.80 mg/L after exposure to Cd in sea-water for 24 and 48 h, respectively, and 2.84, 1.61, and 1.20 mg/L after exposure in freshwater for 24, 48, and 72 h, respectively. The differences in the bioavailability and activity of free Cd2+ caused by the salt concentration in seawater could explain these dramatic differences in the toxicity of Cd between marine and fresh water system. The genes differentially expressed in O. javanicus liver tissue after exposure to 280 mu g/L CdCl2 for 48 h were profiled with a customized marine medaka cDNA microarray (HazChem Fish Array). We identified 204 differentially expressed genes; the expression of 66 genes was upregulated and that of 138 genes was downregulated (P < 0.05). The total 31 genes were commonly expressed in fish exposed to Cd and two references of environmental disruptor (bisphenol A, or 17 beta-estradiol). These genes were used to predict the changes that occur in metabolic pathways and processes in response to Cd exposure. The database for annotation, visualization and integrated discovery (DAVID) was used for functional analysis for the differentially expressed genes. Significant changes were predicted in the steroid hormone and estrogen stimulus response, vitellogenin expression, sterol and cholesterol metabolic processes, lipid transport activity, defense response, innate immune response, and metal ion binding activity. These results extend our knowledge of the toxicity of Cd at the molecular level and indicate that Cd exposure causes endocrine disruption in aquatic organisms.
ISSN
1738-642X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1369
DOI
10.1007/s13273-016-0045-7
Bibliographic Citation
MOLECULAR & CELLULAR TOXICOLOGY, v.12, no.4, pp.409 - 420, 2016
Publisher
KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
Subject
LARGE GENE LISTS; ENVIRONMENTAL SALINITY; JAVANESE MEDAKA; EXPRESSION; BINDING; PROTEIN; 17-BETA-ESTRADIOL; QUALITY; STRESS; METALS
Keywords
Transcriptomic responses; Metabolic changes prediction; Oryzias javanicus; Cadmium; Bisphenol A; 17 beta-Estradiol; Endocrine disrupting chemical
Type
Article
Language
English
Document Type
Article
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse