Searching for novel modes of toxic actions of oil spill using E. coli live cell array reporter system - A Hebei Spirit oil spill study SCIE SCOPUS

Cited 2 time in WEB OF SCIENCE Cited 2 time in Scopus
Title
Searching for novel modes of toxic actions of oil spill using E. coli live cell array reporter system - A Hebei Spirit oil spill study
Author(s)
Jung, Dawoon; Guan, Miao; Lee, Sangwoo; Kim, Cheolmin; Shin, Hyesoo; Hong, Seongjin; Yim, Un Hyuk; Shim, Won Joon; Giesy, John P.; Khim, Jong Seong; Zhang, Xiaowei; Choi, Kyungho
KIOST Author(s)
Yim, Un Hyuk(임운혁)Shim, Won Joon(심원준)
Publication Year
2017-02
Abstract
Oil is a complex mixture of numerous compounds. Therefore, oil spills near shore can cause various adverse effects on coastal ecosystems. However, most toxicological assessments conducted on oil spill sites have focused on limited modes of toxic actions. In the present study, we utilized the Escherichia coli (E. coli) live cell array system (LCA) to identify novel modes of toxicities of the oil spill-affected sediments. For this purpose, sediment samples were collected from an area heavily polluted by Hebei Spirit oil spill (HSOS) incident of 2007. A total of 93 E. coli reporter genes were used to study responses to the chemicals in the mixture. E. coli 1(12 strains were exposed to extracts of oil or the sediment, and changes in gene expression were measured. Exposure to extracts of crude and weathered oil resulted in decreased expression in similar to 30% of tested genes. However, changes in expression observed after exposure to sediment extracts varied. Sediment extracts containing large concentrations of polycyclic aromatic hydrocarbons (PAH) caused down-regulation of >70% of the genes, while extracts containing lesser total concentrations of PAHs exhibited different trends: genes involved in drug resistance were generally up-regulated, while genes responsive to DNA damage were up-regulated in only two extracts. Results suggest that oil pollution can modulate several toxic response pathways related to DNA repair and antibiotic responses. Results from LCA obtained from the sediment and oil samples were different from those observed in the H4IIE-luc assay. Toxicological implications of such observations deserve further examination. Overall, LCA is a promising tool for screening samples and identifying potential modes of toxicities of environmental samples associated with oil spills. (C) 2016 Elsevier Ltd. All rights reserved.
ISSN
0045-6535
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1296
DOI
10.1016/j.chemosphere.2016.11.078
Bibliographic Citation
CHEMOSPHERE, v.169, pp.669 - 677, 2017
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Subject
POLYCYCLIC AROMATIC-HYDROCARBONS; ENDOCRINE-DISRUPTION POTENTIALS; ESCHERICHIA-COLI; COASTAL SEDIMENTS; FLAME RETARDANTS; CRUDE-OIL; MECHANISMS; KOREA; IDENTIFICATION; CLASSIFICATION
Keywords
Live cell array; Environmental stress responsive genes; Sediment; Oil spill
Type
Article
Language
English
Document Type
Article
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse