Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate SCIE SCOPUS

DC Field Value Language
dc.contributor.author Jung, Hae-Chang -
dc.contributor.author Lee, Seong Hyuk -
dc.contributor.author Lee, Sung-Mok -
dc.contributor.author An, Young Jun -
dc.contributor.author Lee, Jung-Hyun -
dc.contributor.author Lee, Hyun Sook -
dc.contributor.author Kang, Sung Gyun -
dc.date.accessioned 2020-04-16T10:25:11Z -
dc.date.available 2020-04-16T10:25:11Z -
dc.date.created 2020-01-28 -
dc.date.issued 2017-07-21 -
dc.identifier.issn 2045-2322 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/1185 -
dc.description.abstract Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H-2. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecular changes. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genome sequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found among the genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formate transporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain, conferred increase in formate consumption and H2 production. Another adaptive passage, carried out by culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth. -
dc.description.uri 1 -
dc.language English -
dc.publisher NATURE PUBLISHING GROUP -
dc.subject THERMOCOCCUS-ONNURINEUS NA1 -
dc.subject BIO-HYDROGEN PRODUCTION -
dc.subject SEA HYDROTHERMAL VENT -
dc.subject ESCHERICHIA-COLI -
dc.subject H-2 PRODUCTION -
dc.subject GENE-CLUSTER -
dc.subject METABOLISM -
dc.subject OVEREXPRESSION -
dc.subject CHALLENGES -
dc.subject OXIDATION -
dc.title Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate -
dc.type Article -
dc.citation.title SCIENTIFIC REPORTS -
dc.citation.volume 7 -
dc.contributor.alternativeName 정해창 -
dc.contributor.alternativeName 이성혁 -
dc.contributor.alternativeName 이성목 -
dc.contributor.alternativeName 안영준 -
dc.contributor.alternativeName 이정현 -
dc.contributor.alternativeName 이현숙 -
dc.contributor.alternativeName 강성균 -
dc.identifier.bibliographicCitation SCIENTIFIC REPORTS, v.7 -
dc.identifier.doi 10.1038/s41598-017-05424-8 -
dc.identifier.scopusid 2-s2.0-85025640045 -
dc.identifier.wosid 000406285700032 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus THERMOCOCCUS-ONNURINEUS NA1 -
dc.subject.keywordPlus BIO-HYDROGEN PRODUCTION -
dc.subject.keywordPlus SEA HYDROTHERMAL VENT -
dc.subject.keywordPlus ESCHERICHIA-COLI -
dc.subject.keywordPlus H-2 PRODUCTION -
dc.subject.keywordPlus GENE-CLUSTER -
dc.subject.keywordPlus METABOLISM -
dc.subject.keywordPlus OVEREXPRESSION -
dc.subject.keywordPlus CHALLENGES -
dc.subject.keywordPlus OXIDATION -
dc.relation.journalWebOfScienceCategory Multidisciplinary Sciences -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Science & Technology - Other Topics -
Appears in Collections:
Marine Resources Research Division > Marine Biotechnology Research Center > 1. Journal Articles
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