Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate SCIE SCOPUS

Cited 6 time in WEB OF SCIENCE Cited 6 time in Scopus
Title
Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate
Author(s)
Jung, Hae-Chang; Lee, Seong Hyuk; Lee, Sung-Mok; An, Young Jun; Lee, Jung-Hyun; Lee, Hyun Sook; Kang, Sung Gyun
KIOST Author(s)
Jung, Hae Chang(정해창)Lee, Seong Hyuk(이성혁)Young, Jun An(안영준)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Alternative Author(s)
정해창; 이성혁; 이성목; 안영준; 이정현; 이현숙; 강성균
Publication Year
2017-07-21
Abstract
Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H-2. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecular changes. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genome sequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found among the genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formate transporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain, conferred increase in formate consumption and H2 production. Another adaptive passage, carried out by culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth.
ISSN
2045-2322
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1185
DOI
10.1038/s41598-017-05424-8
Bibliographic Citation
SCIENTIFIC REPORTS, v.7, 2017
Publisher
NATURE PUBLISHING GROUP
Subject
THERMOCOCCUS-ONNURINEUS NA1; BIO-HYDROGEN PRODUCTION; SEA HYDROTHERMAL VENT; ESCHERICHIA-COLI; H-2 PRODUCTION; GENE-CLUSTER; METABOLISM; OVEREXPRESSION; CHALLENGES; OXIDATION
Type
Article
Language
English
Document Type
Article
Publisher
NATURE PUBLISHING GROUP
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