Effects of various wavelengths of light on physiological stress and non-specific immune responses in black rockfish Sebastes schlegelii subjected to water temperature change

Abstract

We investigated the effects of light-emitting diodes (LEDs) on physiological stress and immunity in black rockfish Sebastes schlegelii subjected to water temperature change [20 (normal temperature) -> 14 -> 20 A degrees C] using progressive stepwise decline [experiment (Exp.) I; 2 A degrees C/day] and direct decline (Exp. II; immediate exposure) in water temperature. We measured stress-related parameters such as cortisol, glucose, heat shock protein 70 and Na+/K+-ATPase, and immune parameters such as immunoglobulin M and lysozyme. We confirmed nuclear DNA damage using a comet assay. Physiological stress indicators were significantly higher, immune parameters were significantly lower, and the amount of nuclear DNA damage was higher after exposure to low temperatures, but these effects were mitigated by exposure to green or blue LEDs. The direct decline in temperature induced more physiological stress and nuclear DNA damage than the progressive stepwise change. Green and blue LEDs aid recovery from physiological damage in fish.