Anti-inflammatory effect of Apo-9′-fucoxanthinone via inhibition of MAPKs and NF-kB signaling pathway in LPS-stimulated RAW 264.7 macrophages and zebrafish model
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Title
- Anti-inflammatory effect of Apo-9′-fucoxanthinone via inhibition of MAPKs and NF-kB signaling pathway in LPS-stimulated RAW 264.7 macrophages and zebrafish model
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Author(s)
- Kim, E.-A.; Kim, S.-Y.; Ye, B.-R.; Kim, J.; Ko, S.-C.; Lee, W.W.; Kim, K.-N.; Choi, I.-W.; Jung, W.-K.; Heo, S.-J.
- KIOST Author(s)
- Kim, Eun A(김은아); Kim, Jun Seong(김준성); Heo, Soo Jin(허수진)
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Alternative Author(s)
- 김은아; 예보람; 김준성; 허수진
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Publication Year
- 2018
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Abstract
- In this study, we confirmed the anti-inflammatory effect of Apo-9-fucoxanthinone (AF) in in vitro RAW 264.7 cells and in vivo zebrafish model. In lipopolysaccharide (LPS)-stimulated zebrafish, AF significantly decreased the production of reactive oxygen species (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of inducible nitric oxide synthase (iNOS), suppressed cyclooxygenase-2 (COX-2) and an inflammatory cytokines; IL-1β TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264.7 cells. Further, AF suppressed COX-2, prostaglandin E2 (PGE2), and pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α) at 25, 50 and 100 μg/mL, respectively. Further mechanistic studies showed that AF suppressed the nuclear factor-kB (NF-kB) pathway and phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). According to the results, AF can be used and applied as a useful anti-inflammatory agent of nutraceutical or pharmaceutical. © 2018 Elsevier B.V.
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ISSN
- 1567-5769
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/1053
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DOI
- 10.1016/j.intimp.2018.03.034
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Bibliographic Citation
- International Immunopharmacology, v.59, pp.339 - 346, 2018
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Publisher
- Elsevier B.V.
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Subject
- algal extract; antiinflammatory agent; apo 9' fucoxanthinone; cyclooxygenase 2; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 1beta; interleukin 6; lipopolysaccharide; mitogen activated protein kinase; mitogen activated protein kinase inhibitor; nitric oxide; nuclear factor; prostaglandin E2; reactive oxygen metabolite; stress activated protein kinase; tumor necrosis factor; unclassified drug; antiinflammatory agent; apo-9' -fucoxanthinone; cyclooxygenase 2; cytokine; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; lipopolysaccharide; mitogen activated protein kinase; nitric oxide; prostaglandin E2; reactive oxygen metabolite; terpene; adult; animal cell; animal experiment; animal model; antiinflammatory activity; Article; cell death; cell viability; concentration response; controlled study; cytokine production; enzyme inhibition; female; macrophage; male; MAPK signaling; mouse; nonhuman; priority journal; prostaglandin synthesis; protein expression; protein phosphorylation; RAW 264.7 cell line; Sargassum; zebra fish; animal; antagonists and inhibitors; drug effect; genetics; metabolism; nonmammalian embryo; signal transduction; Animals; Anti-Inflammatory Agents; Cyclooxygenase 2; Cytokines; Dinoprostone; Embryo, Nonmammalian; Lipopolysaccharides; Mice; Mitogen-Activated Protein Kinases; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase Type II; RAW 264.7 Cells; Reactive Oxygen Species; Signal Transduction; Terpenes; Zebrafish
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Keywords
- Anti-inflammation; Apo-9′-fucoxanthinone; Cytokines; Macrophage; Seaweed; Zebrafish
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Type
- Article
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Language
- English
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Document Type
- Article
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