Identification of anaerobic thermophilic Thermococcus dominant in enrichment cultures from a hydrothermal vent sediment of Tofua arc SCOPUS KCI

Title
Identification of anaerobic thermophilic Thermococcus dominant in enrichment cultures from a hydrothermal vent sediment of Tofua arc
Author(s)
Cha, I.-T.; Park, S.-J.; Kim, J.-G.; Jung, M.-Y.; Kim, S.-J.; Ju, S.-J.; Kwon, K.K.; Rhee, S.-K.
KIOST Author(s)
Kwon, Kae Kyoung(권개경)
Publication Year
2012
Abstract
Hydrothermal vents (HTV) provide special environments for evolution of lives independent on solar energy. HTV samples were gained from Tofua arc trench in Tonga, South Pacific. We investigated archaeal diversity enriched using combinations of various electron donors (yeast extract and H2) and electron acceptors [Iron (III), elemental sulfur (S0) and nitrate. PCR amplification was performed to detect archaeal 16S rRNA genes after the cultures were incubated 65°C and 80°C for 2 weeks. The cultures showing archaeal growth were transferred using the dilution-to-extinction method. 16S rRNA gene PCR-Denaturing Gradient Gel Electrophoresis was used to identify the enriched archaea in the highest dilutions where archaeal growth was observed. Most of cultured archaea belonged to genus of Thermococcus (T. alcaliphilius, T. litoralis, T. celer, T. barossii, T. thoreducens, T. coalescens) with 98-99% 16S rRNA gene similarities. Interestingly, archaeal growth was observed in the cultures with Iron (III) and nitrate as an electron acceptor. It was supposed that archaea might use the elemental sulfur generated from oxidation of the reducing agent, sulfide. To cultivate diverse archaea excluding Thermococcus, it would be required to use other reducing agents instead of sulfide. © 2012, The Microbiological Society of Korea.
ISSN
0440-2413
URI
https://sciwatch.kiost.ac.kr/handle/2019.kiost/3718
DOI
10.7845/kjm.2012.48.1.042
Bibliographic Citation
Korean Journal of Microbiology, v.48, no.1, pp.42 - 47, 2012
Publisher
Microbiological Society of Korea
Keywords
Anaerobic archaea; Enrichment culture; Extinction dilution culture; Hydrothermal vent; Thermococcus
Type
Article
Language
Korean
Document Type
Article
Publisher
Microbiological Society of Korea
Related Researcher
Research Interests

Acquisition of marine microbial bioresources,Marine microbial diversity,Application study on marine microbes,해양미생물 자원 확보,해양미생물 다양성,해양미생물 기능 활용

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